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What is the principle of an autoclave machine ? Working Principal of Autoclave Autoclaves can also be used to take out all kinds of industrial processes and scientific experiments that work best at high heats and pressures. All autoclaves work with a combination of steam, pressure, and time. They operate using principles similar to the common kitchen pressure cooker — that is, the door is locked to form a sealed chamber, and all air within that chamber is replaced by steam. The steam is then pressurized to bring it to the desired sterilization for the desired duration. Let’s explain the autoclave working by three phases of a sterilization cycle: Purge phase: In this phase, steam displaces air within the autoclave chamber and both temperature and pressure begin to increases. Sterilization phase: In this phase autoclaves, harsh conditions destroy bacteria, spores, and other pathogens. Sterilization phase: In this phase autoclaves, harsh conditions destroy bacteria, spores, and other pathogens.

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